TU100 is a naphthoquinone adduct from a 3+2 cycloaddition reaction between N-methyl-4-hydroxylisoquinolinium and 1,4-napthoquinone, following the method established by DiCesare et al. Current research focuses on synthesizing TU100 analogs. The reaction between quinones and N-methylhydroxylisoquinolinium iodides were attempted to produce novel compounds. After extraction, flash column chromatography was employed to separate the product from remaining starting materials, followed by nuclear magnetic resonance (NMR) spectroscopy to assess purity. Thin-layer chromatography (TLC) was frequently used to monitor reaction progress. Expanding the library of TU100 analogs is crucial in getting an analog with optimized biological activity and enhanced potential as chemotherapeutic agents.
