Head louse pediculosis is a widespread public health issue, particularly among school-aged children. This study examines parental knowledge and practices related to louse infestation in Southeast Georgia. A self-administered survey of 201 parents assessed demographic factors, knowledge levels, and behaviors related to head lice. Most parents (94%) resided in Coffee County, with long-term residents demonstrating greater awareness. However, knowledge gaps existed, particularly among highly educated professionals. Socioeconomic and demographic factors appear to influence behaviors, with lower-income and recently moved families more frequently experiencing louse infestation. More respondents from households with shared living spaces, communal activity participation, and reliance on word of mouth for information reported exposure to lice. While over-the-counter treatments were most common, homemade remedies were used by lower-income families. These findings underscore the need for targeted public health interventions to close knowledge gaps, reduce stigma, and promote effective louse prevention and treatment strategies tailored to diverse communities.