Previous studies showed candle burning may contribute to microbial contamination. This study aimed to assess microbial activity around burning synthetic and natural candles in a confined space. Methods: ATP levels, serving as an indicator of microbial activity, were measured using a bioluminescence assay with a luminometer. Measurements were taken at distances of 17, 68, and 125 inches from the burning candles. A one-square-centimeter area of the floor was sampled three times via swabbing, and Relative Light Units (RLU) were recorded. Results: Preliminary findings on microbial activity (RLU/cm²) ranges in the surrounding areas were: (a) before burning: natural candle 15 - 3270; synthetic candle 4 - 60; (b) after burning: natural candle 47 – 1873; synthetic candle 0 – 17. Conclusions: The preliminary results indicate no significant trends in microbial activity on the floor surrounding the two types of burning candles. We expect that future experiments will yield clearer insights.
