This experiment aims to analyze the relationship between gill surface area and oxygen consumption in axolotls under resting conditions. Using intermittent flow respirometry, the rate of oxygen consumption (mgO2/kg/hr) of individual axolotls will be measured to determine their metabolic demands at rest. The gill surface area of each axolotl will be scored based on qualitative analysis of extent of lamellar development and the data will be analyzed to explore how surface area correlates with oxygen consumption. It is hypothesized that axolotls with larger gill surface areas will exhibit higher rates of oxygen consumption due to increased capacity for gas exchange. The results of this study may provide insights into the physiological adaptations of axolotls in relation to respiratory efficiency and metabolic rates.
