The study investigates vitamin C’s impact on Vero cells, a kidney cell line from green monkey, commonly used in vaccine production. Vitamin C (ascorbic acid) is essential for collagen biosynthesis, hydroxylation reactions and free radical scavenging, but humans must obtain it through diet due to inactive gluconolactone oxidase (GULO) gene. Vero cells were cultured in Dulbecco’s modified Eagle medium (DMEM) to examine Vitamin C’s effect on cell attachment and growth. A 10mM of vitamin C stock solution was prepared and MTT assays were conducted to assess cell viability. Preliminary results indicate that Vitamin C significantly inhibits vero cell growth with a notable reduction in proliferation evident at 24 hours and sustained throughout the treatment period. These finding suggest that vitamin C plays a critical role in modulating cellular processes related to immune function and disease prevention.
